Provisional programme
Monday
16
10
h 15 Opening Remarks
10
h 30 Introductory lecture : Evolution: an algorithm for biological
design
Prof. Frances H. ARNOLD, California Institute of Technology,
Pasadena, USA
12
h 00 LUNCH
13
h 00 Session 1 - Creating biodiversity
Chairman
:
Karl JAEGER - Max-Planck-Institut, Mülheim, Germany
13
h 00 : Molecular breeding of enzymes, pathways and organisms
by DNA shuffling
Willem P. STEMMER, Maxygen, Inc. USA
14
h 00 Mining enzymes for industrial biocatalysis from natural
and artificial evolution
Fabrice LEFEVRE, PROTEUS SA, Nimes, France
14
h 30 Combining in vitro and in vivo approaches to generate
optimized combinatorial libraries
Denis POMPON, Centre de Génétique Moléculaire
du CNRS, Gif-sur-Yvette, France
15
h 00 COFFEE BREAK
15
h 30 Session 2 - Screening biodiversity
Chairman
:
Pierre MONSAN - Club CRIN Biotechnologie, Paris, France
15
h 30 From Enzyme assays to catalytic antibodies
Jean-Louis REYMOND, Department of Chemistry & Biochemistry,
University of Bern, Switzerland
16
h 30 Novel developments in high throughput screening for enantioselectivity
Andreas EIPPER, Max-Planck-Institut für Kohlenforschung,
Mülheim an der Ruhr, Germany
17
h 00 Generic Screening Methods: a prerequisite for industrial
biocatalyst development
Marcel WUBBOLTS, DSM Pharmaceutical Products, Geleen, The Netherlands
17
h 30 Screening-based Directed Evolution of Enzymes
Ulrich KETTLING, DIREVO Biotech AG, Nattermannallee 1, Germany
18
h 00 END
18
h 15 EFBIC workshop (China and Europe cooperation) Part 1
18
h 15 Introductory remarks
Brian CLARK, University of Aarhus, Denmark
18
h 30 Cold adaptation of a mesophilic cellulase, EG III from
Trichoderma reesei, by directed evolution
Yinbo QU, State Key Laboratory of Microbial Technology, Shandong
University, Jinan, P. R. China
19
h 00 Enhancement of the activity of L-aspartase and alpha-aspartyl
dipeptidase by directed evolution
Yan FENG, Key Laboratory for Molecular Enzymology and Engineering,
Jilin University, Changchun, China
19
h 30 Discovery and direct evolution of enzymes useful in biotechnology
Yang KEQUIAN, The Institute of Microbiology, Chinese Academy
of Sciences, Beijing, P.R. China
20
h 00 : Genetic encoding of unatural amino acides in proteins
J. TZE-FEI WONG, Department of Biochemistry, Hong Kong University
of Science & Technology, Clear Water Bay, Hong Kong SAR,
China
20
h 30 END
Tuesday
17
8
h 30 Session 3 "Application 1"
Chairman
:
Sven PEDERSEN - Novo Nordisk, Copenhagen, Denmark
8
h 30 Optimization of fungal lipase for diverse applications
Jesper VIND, Protein Design, Novozymes
9
h 30 Directed Evolution of Esterases: Methods for High-throughput
screening
and applications
Uwe T. BORNSCHEUER, Greifswald University, Greifswald, Germany
10
h 00 COFFEE BREAK
10
h 30 Massive mutagenesis: the first High-throughput combinatorial
site
directed mutagenesis
Marc DELCOURT, Biométhodes Evry, France
11 h 00 Modulation of the regioselectivity of a Bacillus a-galactosidase
by directed evolution
Michel DION, Université de Nantes, France
11
h 30 Stability and activity improvement of cephalosporin esterase
EstB
from Burkholderia gladioli by directed evolution
Helmut SCHWAB, Technische Universität Graz, Austria
12
h 00 LUNCH
13
h 00 Poster session
14
h 45 COFFEE BREAK
15
h 00 Session 4 "Application 2"
Chairman
:
Uwe BORNSHEUER - University Greifswald, Germany
15
h 00 Structure-Guided random mutagenesis as an efficient route
to more enantioselective enzymes
Romas J. KAZLAUSKAS, McGill University, Montréal, Québec,
Canada
16
h 00 Engineering outer membrane enzymes for probing structure-function
relations
Maarten R. EGMOND, University of Utrecht, Dept. Membrane Enzymology,
Utrecht, The Netherlands
16
h 30 Residues at the active site of thermostable esterases involved
in catalysis
and substrate specificity
G. MANCO, Istituto di Biochimica delle Proteine, CNR, Naples,
Italy
17
h 00 END
17
h 15 EFBIC workshop (China and Europe cooperation) Part 2
17
h 15 Description of EFBIC and role
David BENNETT, European Federation of Biotechnology
17
h 30 Open discussion about cooperation
19
h 15 END
20
h 00 CONGRESS DINER
Wednesday
18
8
h 30 Session 5 "Application 3"
Chairman
:
Marcel WUBBOLTS - DSM, Geleen, The Netherlands
8
h 30 Creation of enantioselective biocatalysts by directed evolution
Karl-Erich JAEGER, Max-Planck-Institut für Kohlenforschung,
Mülheim, Germany
9
h 30 Protein Engineering by bacterial cell surface display
Harald KOLMAR, Department of Molecular Genetics, Institute for
Microbiology and Genetics, Goettingen, Germany
10
h 00 COFFEE BREAK
10
h 30 Screening for novel enzymes for biocatalytic processes
- facing a quantum leap in accessible molecular diversity
Patrick LORENZ, B.R.A.I.N Aktiengesellschaft, Zwingenberg, Germany
11
h 00 Saturation mutagenesis of toluene ortho-monooxygenase for
naphthalene oxidation and chloroform degradation
Thomas K. WOOD, University of Connecticut, Storrs, USA
11
h 30 Rational evolution approaches to evolved enzymes
K. POWELL, Nautilus Biotech, Evry, France
12
h 00 LUNCH
13
h 00 Poster session
14
h 45 COFFEE BREAK
15
h 00 Session 6 "New developments"
Chairman
:
Giacomo CARREA - CNR Institute, Milano, Italy
15
h 00 Searching sequence space for protein catalysts
Donald HILVERT, Laboratorium für Organische Chemie, Swiss
Federal Institute of Technology (ETH), ETH Hönggerberg,
CH-8093 Zurich, Switzerland
16
h 00 Enzyme engineering by phage display
Jacques FASTREZ, Université Catholique de Louvain, Louvain-la-Neuve,
Belgium
16
h 30 Protein evolution in cell free systems: Affinity, stability,
turnover
Andreas PLÜCKTHUN, Biochemisches Institut, Universität
Zürich, Zürich
17
h 00 In vitro selections of proteins for catalytic activity
using a chemistry of filamentous phages
Jean-luc JESTIN, Institut Pasteur, Paris, France.
17
h 30 Concluding remarks
17
h 45 END
Invited
speakers:
Uwe BORNSCHEUER, Greifswald, Germany
Maarten EGMOND, Unilever, The Netherlands
Jacques FASTREZ, Louvain-La-Neuve, Belgium
Donald HILVERT, Zurich, Switzerland
Karl JAEGER, Bochum, Germany
Romas KAZLAUSKAS, Montreal, Canada
Harald KOLMAR, Göttingen, Germany
Fabrice LEFEVRE, Proteus SA, Nîmes, France
Andreas PLÜCKTHUN, Zurich, Switzerland
Denis POMPON, Paris, France
Manfred REETZ/A. EIPPER, Mülheim/Ruhr, Germany
Jean-Louis REYMOND, Bern, Switzerland
Rolf SCHMID, Stuttgart, Germany
Pim STEMMER, Maxygen, USA
Jesper VIND, Novozymes, Copenhague, Denmark
Marcel WUBBOLTS, DSM, The Netherlands
